phospho src family tyr416 d49g4 rabbit mab (Cell Signaling Technology Inc)

Cell Signaling Technology Inc is a verified supplier

Cell Signaling Technology Inc manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Cell Signaling Technology Inc phospho src family tyr416 d49g4 rabbit mab

A Western blot analysis of the oral squamous carcinoma cell lines to monitor numerous signaling pathways. The phosphorylated and total protein levels were determined as indicated. B Cell viability (%) of the oral squamous carcinoma cell lines treated with each Src inhibitor for 72 h. Viability was measured using CellTiter-Glo, and values are indicated relative to the 1% dimethyl sulfoxide (DMSO) control. Data represent mean ± standard deviation from three independent experiments (n = 3). C IC50 curves of the oral squamous carcinoma cell lines treated with each Src inhibitor for 36 h. Viability was measured using CellTiter-Glo and normalized to that of the 1% DMSO control. Values represent the mean of n = 3. D, E Western blot analysis of the oral squamous carcinoma cell lines to investigate Src (D) and MAPK (E) activity induced by different Src inhibitors. Oral squamous carcinoma cell lines were treated with an Src inhibitor for 18 h, followed by western blot analysis. D indicates the Src phosphorylation <t>(Tyr416),</t> and E reveals MAPK pathway-related proteins. Supplementary Table S1 lists the concentrations of the Src inhibitors used.

Phospho Src Family Tyr416 D49g4 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+phospho+src+tyr416/bio_rxiv__64898__2026__04__02__716058-74-6-39
Average 86 stars, based on 1 article reviews

phospho src family tyr416 d49g4 rabbit mab - by Bioz Stars, 2026-09

86/100 stars

Images

1) Product Images from "Heterogeneous Sensitivity to Src Inhibitors in Oral Squamous Cell Carcinoma and Its Implications for Combination Therapy with Cisplatin"

Article Title: Heterogeneous Sensitivity to Src Inhibitors in Oral Squamous Cell Carcinoma and Its Implications for Combination Therapy with Cisplatin

Journal: bioRxiv

doi: 10.64898/2026.04.02.716058

Figure Legend Snippet: A Western blot analysis of the oral squamous carcinoma cell lines to monitor numerous signaling pathways. The phosphorylated and total protein levels were determined as indicated. B Cell viability (%) of the oral squamous carcinoma cell lines treated with each Src inhibitor for 72 h. Viability was measured using CellTiter-Glo, and values are indicated relative to the 1% dimethyl sulfoxide (DMSO) control. Data represent mean ± standard deviation from three independent experiments (n = 3). C IC50 curves of the oral squamous carcinoma cell lines treated with each Src inhibitor for 36 h. Viability was measured using CellTiter-Glo and normalized to that of the 1% DMSO control. Values represent the mean of n = 3. D, E Western blot analysis of the oral squamous carcinoma cell lines to investigate Src (D) and MAPK (E) activity induced by different Src inhibitors. Oral squamous carcinoma cell lines were treated with an Src inhibitor for 18 h, followed by western blot analysis. D indicates the Src phosphorylation (Tyr416), and E reveals MAPK pathway-related proteins. Supplementary Table S1 lists the concentrations of the Src inhibitors used.

Techniques Used: Western Blot, Protein-Protein interactions, Control, Standard Deviation, Activity Assay, Phospho-proteomics

other:

Article Title: Ptpn21 controls hematopoietic stem cell homeostasis and biomechanics
Article Snippet: Rabbit anti-phospho-Src (Tyr416) , Cell Signaling , Cat# 2101 RRID: AB_331697.

Article Title: α5 integrin regulates hepatic tight junctions through SRC-TET1-mediated DNA hydroxymethylation
Article Snippet: Rabbit anti-phospho-Src (Tyr416) , Cell Signaling Technology , Cat#2101; RRID: AB_331697.

Article Title: Imprecision in Precision Medicine: Differential Response of a Disease-Linked GluN2A Mutant to NMDA Channel Blockers
Article Snippet: Primary antibodies used: rabbit anti-p38 MAPK (Cell Signaling, 9212S, 1:1,000), mouse anti-phospho-p38 MAPK (Cell Signaling, 9216S, 1:1,000), mouse anti-ERK (pan-ERK) (BD Transduction Lab, 610,123, 1:2000), rabbit anti-phospho-p44/42 MAPK (ERK 1/2) (Cell Signaling, 910, 1:1,000), mouse anti-JNK (Santa Cruz, sc-7345, 1:1,000), rabbit anti-pSAPK/JNK (Cell Signaling, 4668S, 1:1,000), rabbit anti-Src (Cell Signaling, 2108S, 1:1,000), rabbit anti-phospho-Src (Tyr416) (Cell Signaling, 2101S, 1:1,000), and mouse anti-β-actin (Sigma, A5441, 1:10,000).

Data-independent acquisition:

Article Title: Evaluation of a Boron-Conjugated SRC Inhibitor Combined with Proton and X-Ray Irradiation in U-87 MG and U-87 MG IDH1 R132H Glioma Cell Lines
Article Snippet: .. The primary antibodies, rabbit anti-IDH (Abcam, Cat# ab172964, RRID: AB_2864315, Cambridge, UK), mouse anti-IDH R132H (Dianova, Cat. #DIA-H09, RRID: AB_2335716, 1:1000, Hamburg, Germany), rabbit anti-Phospho-Src (Tyr416) (Cell Signaling Technology, Cat.# 2101, RRID: AB_331697, 1: 1000, Danvers, MA, USA), and GAPDH (Abcam Cat.# ab181602, RRID: AB_2630358, 1:10,000), were used for wWestern blot. .. The next day, membranes were washed 3 times in 0.1% tween-20 in PBS and then incubated for 1 h at room temperature with the corresponding secondary antibody: goat anti-mouse IgG (H + L) secondary antibody, HRP (Cat.# 31430, Invitrogen, RRID: AB_228307, 1:5000) and anti-rabbit monoclonal antibody HRP (Cat.# 31460, Invitrogen, RRID: AB_228341, 1:10,000, Waltham, MA, USA).

Article Title: Evaluation of a Boron-Conjugated SRC Inhibitor Combined with Proton and X-Ray Irradiation in U-87 MG and U-87 MG IDH1R132H Glioma Cell Lines
Article Snippet: .. The primary antibodies, rabbit anti-IDH (Abcam, Cat# ab172964, RRID: AB_2864315, Cambridge, UK), mouse anti-IDHR132H (Dianova, Cat. #DIA-H09, RRID: AB_2335716, 1:1000, Hamburg, https://doi.org/10.3390/ph19030392 Germany), rabbit anti-Phospho-Src (Tyr416) (Cell Signaling Technology, Cat.# 2101, RRID: AB_331697, 1: 1000, Danvers, MA, USA), and GAPDH (Abcam Cat.# ab181602, RRID: AB_2630358, 1:10,000), were used for wWestern blot. .. The next day, membranes were washed 3 times in 0.1% tween-20 in PBS and then incubated for 1 h at room temperature with the corresponding secondary antibody: goat anti-mouse IgG (H + L) secondary antibody, HRP (Cat.# 31430, Invitrogen, RRID: AB_228307, 1:5000) and anti-rabbit monoclonal antibody HRP (Cat.# 31460, Invitrogen, RRID: AB_228341, 1:10,000, Waltham, MA, USA).

Western Blot:

Article Title: The proto-oncogene tyrosine kinase c-SRC facilitates glioblastoma progression by remodeling fatty acid synthesis
Article Snippet: .. Rabbit anti-SRC (Cat. 2123, 1:1,000 for WB), rabbit anti-phospho-SRC (Tyr416) (Cat. 6943 S, 1:1,000 for WB), mouse anti-Phospho-Tyrosine (Cat. 9411 S, 1:1,000 for WB) mouse anti-β-actin (Cat. A1978, 1:5,000 for WB) and mouse anti-FLAG (Cat. F1804, 1:2,000 for WB) were purchased from Cell Signaling Technology. .. Rabbit anti-ACLY (Cat. 15421-1-AP,1:1,000 for WB), rabbit anti-ACSS2 (Cat.16087-1-AP, 1:1,000 for WB), rabbit anti-HA (Cat. 51064-2-AP, 1:2,000 for WB), and rabbit anti-FLAG (Cat. 20543-1-AP,1: 1,000 for WB) were purchased from Proteintech.

Incubation:

Article Title: Optical regulation of endogenous RhoA reveals selection of cellular responses by signal amplitude
Article Snippet: .. Cells were incubated in 5% BSA in PBS for 1 hr, and then with rabbit anti-phospho Src Tyr416 (0.69 μg/mL, #2101, Cell Signaling) for overnight at 4°C. .. After three times of washing with PBS, cells were incubated with goat anti-rabbit antibody conjugated to Alexa-fluor 594 (20 μg/mL, #A-11037, Thermo Fisher Scientific) for 1 hr.

Review